Thymosin Beta-4 (TB-500) Peptide Complete Profile, Dosage & Usage

Many individuals who only casually go to the gym are unaware of the severe impact injuries can have on serious athletes and bodybuilders. For this, sustaining an injury can result in significantly more suffering than the average person. To minimize the physical and mental toll of injuries on bodybuilders, these individuals may turn to compounds like TB-500, which have been found to possess quick healing and anti-inflammatory properties based on evidence from human reviews.

TB-500 is a lab-made version of a protein called Thymosin Beta-4, which has demonstrated numerous health benefits and healing properties. Bodybuilders, in particular, may find TB-500 helpful in maximizing their training results. Animal racers have used it to improve performance and speed up injury recovery, and the same benefits could apply to humans. However, weighing the advantages and disadvantages of using TB-500 before deciding to use it is crucial.

Below is the detailed article on TB-500 Peptide Profile. 

What Is TB-500?

Tb-500 Vial

TB-500 is a synthetic peptide derived from Thymosin Beta 4 (TB4), a naturally occurring healing protein found in almost all human and mammalian cells. This peptide is believed to positively impact various aspects of wound healing, injury recovery, inflammation, and flexibility. According to research, TB-500's effects are primarily on blood cell and blood vessel development, cellular differentiation, and cell migration. The peptide is particularly effective because it regulates actin, a critical cell-building protein for wound repair and healing.

The thymus gland produces TB-500, and wound fluids contain it in high concentrations. Studies have demonstrated its effectiveness in wound healing and repair of skin, cornea, and heart by facilitating the creation of new blood vessels, muscle tissue fibres, and blood cells and cell migration. For individuals experiencing stress and injury, TB-500 can provide highly desirable wound-healing effects. TB-500 is also naturally present in high concentrations in blood platelets.

TB 500 background information

As previously mentioned, TB-500 is a man-made variant of Thymosin Beta 4, a protein peptide naturally present in animals and humans. Although it is available for research purposes, TB-500 is commonly administered by veterinarians in clinical trials on horses to promote healing and shorten recovery time after injuries. Research on the effects of thymosin on horses dates back to the 1960s when Dr Allan Goldstein developed thymosin alpha 1 to enhance immune cell activity and later formulated thymosin beta 4 (TB-500) to accelerate wound healing and repair.

Studies have demonstrated that TB-500 can significantly reduce inflammation and inhibit adhesion formation following injuries in horse racing. Thymosin was first used in humans in 1974 when a young girl with a non-functioning thymus gland received thymosin injections. Since then, many athletes and biohackers have been using thymosin for its reparative effects, ability to reduce inflammation, and expedite recovery.

TB-500 - action

TB-500 is highly valued by athletes for its ability to accelerate tissue repair and healing processes, almost to a clinical level. However, TB-500 has a pleiotropic effect on the entire body. It stimulates the production of T lymphocytesregulates actin levels, and increases the number of B cells, enhancing users' overall immunity. Additionally, it promotes angiogenesis by building new blood vessels, supplying oxygen to muscles, and maintaining the elasticity of tendons and ligaments, which can help prevent injuries. Interestingly, TB-500 is also sometimes used as a supportive element for excessive hair loss.

Benefits Of TB-500

Benefits Of TB-500

TB-500 has many benefits, focusing on quicker wound healing and muscle growth. Some of the major benefits of TB-500 are as follows:

  • Rapid wound healing: TB-500 accelerates the healing process of wounds and injuries by promoting angiogenesis, cell proliferation, and regeneration pathways. It also helps to degrade enzymes and promotes cell migration, particularly around muscles.
  • Heal's ligament and joint injuries: TB-500 evenly spaces out collagen fibres, which aids in the healing of ligament injuries. It also speeds up joint recovery when consumed in adequate concentrations.
  • Regenerates brain function: TB-500 influences brain regeneration to minimize the decline in brain functioning. It improves the working of the nervous system in several different ways, which enhances behaviours, cognitive capacities, and neuronal health, and diminishes pain experienced during nervous disorders.
  • Improves cardiac health: TB-500 allows the healthy growth of heart cells, regenerates heart tissue, and reduces the chances of damage. It also has healing effects on patients suffering from myocardial infarctions and strokes.
  • Helps build endurance: TB-500 enhances muscle endurance due to quicker healing, reduced inflammation, and proliferative growth. Greater endurance leads to a high stamina level, essential for athletes and bodybuilders.
  • Stimulates hair growth: TB-500 regulates keratinocytes, stimulating hair follicles and promoting hair growth.

How Does It Work?

Bodybuilder injecting TB-500

TB-500 facilitates the upregulation of cellular proteins responsible for tissue building and repair, resulting in increased productivity and improved responsiveness to hormones involved in these processes. Of these hormones, actin plays a crucial role by promoting cellular tissue growth and reproduction, allowing for the replacement of damaged tissue with healthy new cells. Actin also aids in forming new blood vessels and positive inflammation, both important factors in enhancing immune cell access to injured tissue. TB-500's unique molecular structure enables it to travel effortlessly throughout the body and target the injured tissue specifically for therapeutic intervention.

How To Use TB-500?

TB-500 can be given intramuscularly, intravenously, or subcutaneously. Mix the TB-500 powder with bacteriostatic water to the necessary concentration to make the injectable solution. After preparing the solution, wipe the injection site with an alcohol swab, and draw and inject the required volume using the specified administration route.

The intramuscular approach is the most widely used. Intravenous injection is a more medically supervised procedure that involves identifying the correct vein and injecting the fluid directly into it.

TB-500 Cycling

There are two phases for cycling. The first phase, which is the initial phase, lasts 2 to 6 weeks. It is divided into multiple doses, like twice or twice a week.
The second step, also called the maintenance phase, last for a 2 to 6 mg reduction every two weeks. 

What Is The Recommended Dosage?

Tb-500 Vials

Keep the TB-500 for 4 to 10 mg twice weekly during the first month until the body adjusts. After this, the dosage can be reduced to 2 to 5 mg once a week for a few weeks before being taken once a month. 

What Are The Side Effects?

TB-500 has some adverse effects when used regularly. 

  • It can cause colon cancer. 
  • It also causes patients with cancer to have a higher concentration of thymosin, the chemical from which TB-500 is generated.
  • It can also cause lightheadedness or nausea; others may even cause flu-like symptoms.
  • It also causes irritation at the injection site. 

Is TB-500 Legal?

TB-500 is considered illegal by WADA and is strictly prohibited in sports. This means that athletes are allowed to use it. As it is used to enhance performance, using TB-500 can disqualify or penalize athletes. 


TB-500 is a peptide that can help with the recovery of both acute and chronic injuries, as well as the treatment of tendinitis. It can also increase flexibility when it has been compromised by injury and has partially regrown lost hair in some circumstances. 

It has numerous possible advantages. It helps with wound healing, hair loss, and various other issues. TB 500 acts by stimulating the development of new blood vessels and increasing the production of extracellular matrix proteins. This promotes tissue healing and the development of new cells. If you're looking for a peptide with various potential benefits, TB 500 is an excellent choice. 

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